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1.
Vitae (Medellín) ; 19(3): 287-293, sep.-dic. 2012. ilus, tab
Article in English | LILACS | ID: lil-669354

ABSTRACT

background: Lactic acid (LA) is a carboxylic acid widely used as preservative, acidulant, and/or flavouring in food industry; it is also used as a raw material for the production of lactate ester, propylene glycol, 2,3-pentanedione, propanoic acid, acrylic acid and acetaldehyde. In recent years, the demand for LA production has dramatically increased due to its application as a monomer for poly-lactic acid synthesis, a biodegradable polymer used as a plastic in many industrial applications. LA can be produced either by fermentation or chemical synthesis; the former route has received considerable interest, due to environmental concerns and the limited nature of petrochemical feedstocks; thus, 90% of LA produced worldwide is obtained by fermentation, this process comprises the bioconversion of a sugar solution (carbohydrates) into LA in the presence of a microorganism. Objectives: This work is aimed at studying the effect of pH control and culture media composition on the LA production using renewable sources from the agroindustry sector. Methods: A Lactobacillus brevis strain is used to perform lab scale experiments under aerobic and anaerobic conditions, using three different culture media compositions: a high nutritional content medium (MRS), as a reference, a low nutritional content medium with glucose as the only carbon source (GM), and a potential low nutritional content medium with cassava flour as carbon source (HY1). results: The higher LA production is accomplished under anaerobic conditions, 17.6 ± 0.1, 12.6 ± 0.2 y 13.6 ± 0.2 g LA/L, for MRS, GM and HY1 medium, respectively. The effect of pH on LA biosynthesis in a 5L bioreactor is also studied using the HY1 medium. For a fermentation time of 120 h, the highest LA concentration obtained was 24.3 ± 0.7g LA/L, productivity 0.20 g/L/h, YP/S 0.32g LA/g syrup, at pH 6.5...


Subject(s)
Levilactobacillus brevis , Lactic Acid
2.
Vitae (Medellín) ; 15(1): 25-31, jan.-jun. 2008. mapas, tab
Article in Spanish | LILACS | ID: lil-502217

ABSTRACT

Las microalgas son consideradas alimentos funcionales, capaces no sólo de elevar el contenido nutricional de los alimentos tradicionales, sino también de afectar positivamente la salud de animales y humanos, debido a que contienen cantidades apreciables de proteínas, vitaminas y ácidos grasos poliinsaturados. Algunas microalgas incluso tienen un contenido aminoacídico superior al presentado por alimentos convencionales, tal es el caso de Scenedesmus sp que posee niveles de lisina superiores al patrón de la Food and Agriculture Organization of the United Nations (FAO), y niveles de proteína entre 25 y 35 por ciento lo que la hace una atractiva fuente de proteína. Por lo tanto, en este trabajo se busca el medio de cultivo donde se pueda alcanzar la mayor producción de biomasa y proteína de la microalga verde Scenedesmus sp, evaluando los medios de cultivo Estándar 1, Estándar 2 y Algal y, empleando, NaHCO3 como fuente de carbono. El medio Estándar 2 presenta la mayor producción de biomasa (0.41 g/ L) y el medio Algal, el mas alto porcentaje de proteína por materia seca (35.18 por ciento). Adicionalmente, se estudia el polimorfismo presentado por la microalga en cada medio de cultivo, y se hacen evidentes cambios morfológicos, incluso entre colonias con el mismo número de células. Esta microalga tiene aplicación potencial en la industria de alimentos balanceados para animales


Subject(s)
Eukaryota , Biomass
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